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0:47
YouTube
NCSU BIT
Demo of Agrobacterium Infiltration
Demonstration of Agrobacterium infiltration into tobacco leaves using a flat-tipped 1 mL syringe barrel. Performed by Dr. Melissa Srougi. Method: 1) To infiltrate leaves, suck up Agrobacterium solution with the 1mL syringe barrel. 1.1) Infiltration solution is made from 10 mM MES buffer pH 5.6 and 10 mM MgCl2 and used to suspend pelleted ...
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